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Recombinant DNA Technology

Isolation of DNA, Fragmentation of DNA by restriction endonucleases...

Process of Recombinant DNA Technology

Recombinant DNA technology involves the following steps:

  1. Isolation of DNA.

  2. Fragmentation of DNA by restriction endonucleases.

  3. Isolation of a desired DNA fragment.

  4. Amplification of the gene of interest.

  5. Ligation of the DNA fragment into a vector.

  6. Insertion of recombinant DNA into the host.

  7. Culturing the host cells on a suitable medium at a large scale.

  8. Extraction of the desired gene product.

  9. Downstream processing of the products as finished product, ready for marketing.

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